How to interpret a heatmap for differential gene expression analysis - simply explained!

Biostatsquid • April 12, 2023
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Biostatsquid

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Welcome to Biostatsquid! My name is Laura, I am a bioinformatician who loves finding new ways to analyse and visualise biological data - from scRNAseq to proteomics! I started this channel as a way to share my experiences, teach but also learn from others! Coding and stats can be as (or more!) fun as lab work, and everyone can learn how to do it! In this channel you'll find explanations and hands-on tutorials for analysing and interpreting biological data. - Learn basic techniques in biostatistics, from heatmaps to pathway enrichment analysis. - Manage your data and create publication-ready visualisations by following my easy step-by-step tutorials in R and Python. - Practical explanations without complex formulas for you to understand your datasets and be able to interpret your results. What are you waiting for? Just dive in!

Video Description

In this video, I will focus on how to interpret a heatmap for differential gene expression analysis. Learn why heatmaps are a great visualisation tool for our DEG or gene set enrichment analysis results, why clustered heatmaps help us find patterns in our genes and/or samples and how to interpret a heatmap from a real-life published example! We will go through heatmaps from the very basics and then apply the main points to interpret a heatmap from a scientific paper. Hope you like it! -------------------------------------------------------------------------------------------------------------------- Watched it already? If you liked this video or found it useful, please let me know! Your comments and feedback are very much appreciated😊 If you have questions, don't hesitate to leave me a comment down below, I will answer as soon as I can:) -------------------------------------------------------------------------------------------------------------------- Are you into biostatistics and computational analysis? For more biostatistics tools and resources, you can visit: https://biostatsquid.com/ Follow me on Instagram at @biostatsquid: https://www.instagram.com/biostatsquid/ For more • simple and clear explanations of biostatistics methods • computational biology tools • easy step-by-step tutorials in R and Python to analyse and visualise your biological data! Don’t forget to subscribe if you don’t want to miss another video from me! -------------------------------------------------------------------------------------------------------------------- Other interesting resources for heatmaps: Some additional slides and tips when creating a heatmap: https://acikders.ankara.edu.tr/pluginfile.php/189082/mod_resource/content/0/heatmap.pdf Don't want to code? You can easily create your publication-ready heatmaps with this RShiny App: http://shinyheatmap.com/ or if you want to program your way through it, I recommend the pheatmap packages (planning on a tutorial soon, but Dave Tang has an amazing step by step guide!): https://davetang.org/muse/2018/05/15/making-a-heatmap-in-r-with-the-pheatmap-package/ https://bioconductor.org/packages/release/bioc/html/clusterProfiler.html

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