Video Description

In this tutorial, I will explain how to perform pathway enrichment analysis on your differential gene expression analysis results. We will use the R package clusterProfiler() and you will learn how to: - Download and use gene sets in the context of PEA - Prepare your differential gene expression results for PEA - Perform functional enrichment analysis with clusterProfiler() - and interpret the results! In part 2, we will explore various plots to visualise your results! https://youtu.be/TWiN_qNmYX0 And as always, you can find the code I am using in this tutorial at biostatsquid.com, where you can also find a step by step explanation of the code. For this tutorial you will need R, or Rstudio, and you will need to install the clusterProfiler() package. Hope you like it! Code explained: https://biostatsquid.com/pathway-enrichment-analysis-tutorial-clusterprofiler/ -------------------------------------------------------------------------------------------------------------------- Watched it already? If you liked this video or found it useful, please let me know! Your comments and feedback are very much appreciated😊 If you have questions, don't hesitate to leave me a comment down below, I will answer as soon as I can:) -------------------------------------------------------------------------------------------------------------------- Are you into biostatistics and computational analysis? For more biostatistics tools and resources, you can visit: https://biostatsquid.com/ Follow me on Instagram at @biostatsquid: https://www.instagram.com/biostatsquid/ For more • simple and clear explanations of biostatistics methods • computational biology tools • easy step-by-step tutorials in R and Python to analyse and visualise your biological data! Don’t forget to subscribe if you don’t want to miss another video from me! -------------------------------------------------------------------------------------------------------------------- Other interesting resources for pathway enrichment analysis: clusterProfiler package: https://bioconductor.org/packages/release/bioc/html/clusterProfiler.html clusterProfiler publication: https://www.sciencedirect.com/science/article/pii/S2666675821000667 More on Pathway Enrichment Analysis: https://biostatsquid.com/pathway-enrichment-analysis-explained/ Dataset from: https://rdrr.io/github/LTLA/scRNAseq/man/BacherTCellData.html Music from #Uppbeat (free for Creators!): https://uppbeat.io/t/pecan-pie/east-side-horns License code: 80ZOLNQ8NSDSWKFV